Aim: We aimed to investigate the prevalence and molecular characterization of rabies virus (RABV) from wild and domestic animals in Mongolia during 2008-2010.

Materials and Methods: Brain tissue samples were collected from 24 rabid animals in Zavkhan, Omnogovi, Tov, Dundgovi, Govi-Altai, Selenge, Ovorkhangai, and Khentii provinces in Mongolia. Herein, samples were included from 13 domestic animals (dogs, cattle, camels, sheep, and goat) and 11 wild animals (wolves and foxes) in this study. Direct fluorescent antibody (DFA) test and reverse transcriptase polymerase chain reaction (RT-PCR) were performed for detection of RABV, and positive samples were further processed for molecular characterization of the virus using nucleoprotein gene. Subsequently, the molecular characterization was determined based on the nucleoprotein gene.

Results: Out of 24 samples, 22 samples were detected positive for RABV by DFA test, and its nucleoprotein gene was amplified in all of the 24 samples by RT-PCR. These Mongolian RABVs were classified within steppe-type virus clade by phylogenetic analysis of nucleoprotein gene sequences. This steppe-type virus clade was clearly divided by two Sub-clades (A and B). The most of Mongolian RABVs belongs to the Sub-clade A in the phylogenetic tree.

Conclusion: These findings have clearly confirmed RABV in domestic and wild animals of Mongolia. Further molecular characterization indicated that this Mongolian strain is steppe-type virus clade consisting of two sub-clades; the Subclade A might be prevalent in Altai, Khangai, Khentii Mountains as a major genotype, whereas the Subclade B seems to be cosmopolitan in the steppe-type virus clade, is spread in northern central Eurasia.

Keywords: detection, molecular characterization, Mongolia, rabies virus, sub-clade A, subclade B.

1. Yang J, Hooper DC, Wunner WH, Koprowski H, Dietzschold B, Fu ZF. The specificity of rabies virus RNA encapsidation by nucleoprotein. Virology 1998;242:107-17. [Crossref] [PubMed]

2. Kissi B, Tordo N, Bourhy H. Genetic polymorphism in the rabies virus nucleoprotein gene. Virology 1995;209:526-37. [Crossref] [PubMed]

3. World Health Organization. WHO Expert Consultation on Rabies. Second report. World Health Organ Tech Rep Ser 2013;1-139.

4. Botvinkin AD, Otgonbaatar D, Tsoodol S, Kuzmin IV. Rabies in the Mongolian steppes. Dev Biol (Basel) 2008;131:199-205.

5. Odontsetseg N, Uuganbayar D, Tserendorj SH, Adiyasuren Z. Animal and human rabies in Mongolia. Rev Sci Tech 2009;28:995-1003. [PubMed]

6. Boldbaatar B, Inoue S, Tuya N, Dulam P, Batchuluun D, Sugiura N, et al. Molecular epidemiology of rabies virus in Mongolia, 2005-2008. Jpn J Infect Dis 2010;63:358-63. [PubMed]

7. Kuzmin IV, Botvinkin AD, McElhinney LM, Smith JS, Orciari LA, Hughes GJ, et al. Molecular epidemiology of terrestrial rabies in the former Soviet Union. J Wildl Dis 2004;40:617-31. [Crossref] [PubMed]

8. Wang L, Tang Q, Liang G. Rabies and rabies virus in wildlife in mainland China, 1990-2013. Int J Infect Dis 2014;25:122-9. [Crossref] [PubMed]

9. Zhang HL, Zhang YZ, Yang WH, Tao XY, Li H, Ding JC, et al. Molecular epidemiology of reemergent rabies in Yunnan Province, southwestern China. Emerg Infect Dis 2014;20:1433-42. [Crossref] [PubMed] [PMC]

10. Yin JF, Wang JL, Tang Q, Ding YL, Tao X, Li H, et al. Identification of animal rabies in Inner Mongolia and analysis of the etiologic characteristics. Biomed Environ Sci 2014;27:35-44. [PubMed]

11. Feng Y, Wang W, Guo J, Alatengheli, Li Y, Yang G, et al. Disease outbreaks caused by steppe-type rabies viruses in China. Epidemiol Infect 2015;143:1287-91. [Crossref] [PubMed]

12. Heaton PR, Johnstone P, McElhinney LM, Cowley R, O'Sullivan E, Whitby JE. Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses. J Clin Microbiol 1997;35:2762-6. [PubMed] [PMC]

13. Campanella JJ, Bitincka L, Smalley J. MatGAT: An application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinformatics 2003;4:29. [Crossref] [PubMed] [PMC]

14. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725-9. [Crossref] [PubMed] [PMC]

15. Tamura K. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G C-content biases. Mol Biol Evol 1992;9:678-87. [PubMed]