Molecular genotypes analysis of Cryptosporidium and Hymenolepis in rats on Lombok Island, Indonesia

Research (Published online: 08-09-2020)

5. Molecular genotypes analysis of Cryptosporidium and Hymenolepis in rats on Lombok Island, Indonesia

Ersandhi Resnhaleksmana, Wayan Tunas Artama, Mahardika Agus Wijayanti and Fihiruddin Fihiruddin

International Journal of One Health, 6(2): 123-127

Ersandhi Resnhaleksmana: Doctoral Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Wayan Tunas Artama: Department of Biochemistry, Faculty of Veterinary Medicine, and One Health/Ecohealth Resource Center, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Mahardika Agus Wijayanti: Department of Parasitology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Fihiruddin Fihiruddin: Department of Medical Laboratory Technology, Politeknik Kesehatan Mataram, Indonesia.

doi: www.doi.org/10.14202/IJOH.2020.123-127

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Article history: Received: 10-06-2020, Accepted: 04-08-2020, Published online: 08-09-2020

Corresponding author: Ersandhi Resnhaleksmana

E-mail: resnha@gmail.com

Citation: Resnhaleksmana E, Artama WT, Wijayanti MA, Fihiruddin F (2020) Molecular genotypes analysis of Cryptosporidium and Hymenolepis in rats on Lombok Island, Indonesia, Int. J. One Health, 6(2): 123-127.

Abstract

Background and Aim: Cryptosporidium parvum and Hymenolepis nana are intestinal parasites that are commonly found in the unclean environment. Their presence in rats promotes the transmission of the cryptosporidiosis and hymenolepiasis to humans or animals nearby. This study aimed to determine the molecular characteristics of C. parvum and H. nana and their distribution in rats on Lombok Island.

Materials and Methods: C. parvum and H. nana were investigated in 50 rats from Lombok Island. The molecular-parasitological technique used was polymerase chain reaction and sequencing method.

Results: From 50 samples of rats' stool from 10 locations on Lombok Island, 8% (4/50) of C. parvum was detected molecularly with an 18S rRNA gene and 2% (1/50) of H. nana with COX 1 gene. Phylogenetic analysis indicated that C. parvum carrying rats on Lombok Island have a genetic relationship with C. parvum with Obi7 isolates, Japan and H. nana has a genetic relationship with Rodentolepis nana identified with Hn-VT isolates, India.

Conclusion: The highest incidence of parasites was found in rats that were caught in the urban areas of Lombok Island, West Nusa Tenggara, Indonesia. Immediately, public health programs in these types of contaminated areas should receive priority attention to prevent further transmission of the parasites from animals to human beings.

Keywords: Cryptosporidium, Hymenolepis, intestinal parasite, Rattus.