In vitro trichomonocidal potency of Naja nigricollis and Bitis arietans snake venom

Research (Published online: 16-02-2021)

2. In vitro trichomonocidal potency of Naja nigricollis and Bitis arietans snake venom

Tijjani Sabiu Imam, Zainab Tukur, Aisha Abdullahi Bala, Nuraddeen Bello Ahmad and Adamu Yunusa Ugya

International Journal of One Health, 7(1): 6-11

Tijjani Sabiu Imam: Department of Biological Sciences, Bayero University Kano, Kano, Nigeria.

Zainab Tukur: Department of Biological Sciences, Bayero University Kano, Kano, Nigeria.

Aisha Abdullahi Bala: Department of Biological Sciences, Bayero University Kano, Kano, Nigeria.

Nuraddeen Bello Ahmad: Department of Biological Sciences, Kaduna State University, Kaduna State, Nigeria.

Adamu Yunusa Ugya: Key Lab of Groundwater Resources and Environment of Ministry of Education, Key Lab of Water Resources and Aquatic Environment of Jilin Province, College of New Energy and Environment, Jilin University, Changchun, China; Department of Environmental Management, Kaduna State University, Kaduna, Nigeria.

doi: www.doi.org/10.14202/IJOH.2021.6-11

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Article history: Received: 05-10-2020, Accepted: 25-01-2021, Published online: 16-02-2021

Corresponding author: Adamu Yunusa Ugya

E-mail: ugya88@kasu.edu.ng

Citation: Imam TS, Tukur Z, Bala AA, Ahmad NB, Ugya AY (2021) In vitro trichomonocidal potency of Naja nigricolis and Bitis arientans snake venom, Int. J. One Health, 7(1): 6-11.

Abstract

Background and Aim: Trichomonas vaginalis drug's limited efficacy and high toxicity, justify the need to explore other therapeutic agents, including animal toxins. In this study, the Naja nigricollis and Bitis arietans snake venoms were used to assess such trichomonocidal effect.

Materials and Methods: The median lethal dose (LD₅₀) value for both snake species was calculated by probit analysis using a statistical package for the sciences version 20.0 with an LD₅₀ of 4.04 μg/mL for the N. nigricollis, and no mortality was observed in the B. arietans envenomed rats.

Results: The trichomonocidal potency of the snake venom on T. vaginalis was evident with a growth inhibitory concentration of 89% with a half-maximal inhibitory concentration (IC₅₀) of 0.805 μg/mL in B. arietans while 95% for N. nigricollis at an IC₅₀ of 0.411 μg/mL.

Conclusion: The statistical analysis of one-way analysis of variance shows a significant difference (p<0.05) between the venoms and positive control group (p<0.001), and there is no significant difference between each venom and its varying concentration (p>0.05). As the least concentration can be useful, interestingly, there is no significant difference in the efficacy of N. nigricollis and B. arietans to T. vaginalis (p>0.05); as such, either of the venom can be used for the treatment of trichomoniasis.

Keywords: Bitis arietans, Naja nigricollis, Trichomonas vaginalis, trichomonocidal activities.