Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh

Research Article

International Journal of One Health, 8(2): 101-107

https://doi.org/10.14202/IJOH.2022.101-107

Talal Hossain¹, Md. Abu Sayem Khan¹, Md. Firoz Ahmed², and Sabita Rezwana Rahman¹

1. Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
2. Department of Microbiology, Jahangirnagar University, Savar, Dhaka, Bangladesh.

Background and Aim: Salmonella spp. are frequently associated with various parts of the egg, including the shell, and cause foodborne outbreaks worldwide. Antibiotic-resistant Salmonella spp. pose serious threats to human and animal health; therefore, preventive measures against these pathogens are important. This study aimed to isolate and characterize Salmonella spp. from eggshell samples from different local markets in Dhaka, Bangladesh.

Materials and Methods: Salmonella spp. were recovered from eggshells by enrichment culture and biochemical tests and characterized through molecular amplification of Salmonella-specific genes. Antibiotic sensitivity testing and molecular detection of isolates were performed by disk diffusion method and polymerase chain reaction (PCR), respectively. The invA, fliC, and sdfI genes were used in PCR to identify the genus Salmonella, and the species Salmonella Typhimurium and Salmonella Enteritidis, respectively.

Results: The prevalence of Salmonella spp. was recorded as 40%, in which S. Typhimurium was the predominant serotype. PCR analysis revealed that 100%, 59%, and 13.6% of these isolates possessed the invA, fliC, and sdfI genes, respectively. The isolates exhibited multidrug resistance phenotypes, with resistance (95.5%) toward tetracycline, sulfamethoxazole, and clindamycin and sensitivity (86.3%) toward chloramphenicol.

Conclusion: The findings of this study reflect the potential of eggs as a reservoir of multidrug-resistant Salmonella spp.; therefore, we recommend the careful handling of eggs to avoid contamination from farm to market. Keywords: antibiotic resistance, eggs, health hazards, poultry industry, Salmonella.

Keywords: antibiotic resistance, eggs, health hazards, poultry industry, Salmonella.

How to cite this article: Hossain T, Khan MAS, Ahmed MF, and Rahman SR (2022) Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh, Int. J. One Health, 8(2): 101–107.

Received: 07-04-2022 Accepted: 09-09-2022 Published online: 07-11-2022

Corresponding author: Sabita Rezwana Rahman E-mail: sabita_rahman@hotmail.com

DOI: 10.14202/IJOH.2022.101-107

Copyright: Hossain, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.