Research Article | 23 Dec 2019

Treatment of experimentally induced diabetic wound infected with methicillin-resistant Staphylococcus aureus using Aloe vera, Apium graveolens, and Sauropus androgynus extracts in rats

Yos Adi Prakoso1,2, Kurniasih Kurniasih3, Agustina Dwi Wijayanti4, and Yuli Purwandari Kristianingrum3Show more
1. Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, University of Gadjah Mada, Yogyakarta 55281, Indonesia.
2. Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, East Java 60225, Indonesia.
3. Department of Pathology, Faculty of Veterinary Medicine, University of Gadjah Mada, Yogyakarta 55281, Indonesia.
4. Department of Pharmacology, Faculty of Veterinary Medicine, University of Gadjah Mada, Yogyakarta, 55281, Indonesia.

Corresponding author: Kurniasih Kurniasih (kurniasih_1951@yahoo.co.id)

Received: 23-07-2019, Accepted: 13-11-2019, Published: 23-12-2019

INTERNATIONAL JOURNAL OF ONE HEALTH | pg no. 99-106 | Vol. 5, Issue 1 | DOI: 10.14202/IJOH.2019.99-106
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Abstract

              
Background and Aim: One of the complications of diabetes mellitus is diabetic ulcer. Diabetic ulcer is commonly infected by infectious agents, especially methicillin-resistant Staphylococcus aureus (MRSA). This study aimed to evaluate the potential effects of alcoholic extracts of Aloe vera, Apium graveolens, and Sauropus androgynus on promoting wound healing in a diabetic wound infected with MRSA.
Materials and Methods: A total of 60 male Sprague-Dawley rats (6 months old, weighing 250-300 g) were injected with 65 mg/kg body weight of streptozotocin to induce diabetes. On day 7, the backs of the rats were shaved, and two circular wounds (4 mm in diameter) were created on their back, which were infected with MRSA. The rats were divided into six groups: Group I = control, Group II = treated with cream base without extract, Group III = treated with 2% A. vera cream, Group IV = treated with 2% A. graveolens cream, Group V = treated with 2% S. androgynus cream, and Group VI = treated with 2% A. vera + 2% A. graveolens + 2% S. androgynus cream. The wounds were treated twice a day for 14 days. The data were collected on days 7 and 14.
Results: The results showed that all three herbal extracts and their combination decreased wound area and percentage of the wound, increased tensile strength of skin, collagen deposition, vascular endothelial growth factor expression, and skin thickness, and depressed the C-reactive protein profile and cyclooxygenase-2 expression.
Conclusion: A. vera, A. graveolens, and S. androgynus creams can be used as herbal therapies against diabetic wounds infected with MRSA, both as a single and combination treatment. Keywords: Aloe vera, Apium graveolens, diabetes ulcer, methicillin-resistant Staphylococcus aureus, Sauropus androgynus.

             
Keywords: Aloe vera, Apium graveolens, diabetes ulcer, methicillin-resistant Staphylococcus aureus, Sauropus androgynus.