Coresistance to quaternary ammonium compounds in extended-spectrum beta-lactamase-producing Escherichia coli

Research Article

International Journal of One Health, 6(2): 134-142

https://doi.org/10.14202/IJOH.2020.134-142

Susanne Sütterlin¹, Anna Heydecke², and Eva Tano³

1. Department of Women's and Child's Health, International Maternal and Child Health, Uppsala University, Uppsala, Sweden.
2. Center for Research and Development Gävleborg, Uppsala University, Gävle, Sweden.
3. Department of Medical Sciences, Uppsala University, Uppsala, Sweden.

Background and Aim: Extended-spectrum β-lactamases (ESBL) in Escherichia coli constitutes one of the major threats to modern medicine, and the increasing pollution with quaternary ammonium compounds (QACs) has been suspected to contribute to the spread of ESBL-producing bacteria. The aim of the study was to investigate ESBLA and ESBLM-C-producing E. coli isolates for their coresistance to QACs and their phylogeny isolated from a Swedish University Hospital.

Materials and Methods: Coresistance in E. coli with production of ESBL enzymes of the type blaCTX-M (n=23) was compared to E. coli producing AmpC type ESBL enzymes blaCMY and blaDHA (n=27). All isolates were tested for susceptibility to antibiotics and QACs, and high-quality whole-genome sequences were analyzed for resistance determinants.

Results: The plasmid-borne small multidrug resistance (SMR) efflux pump sugE(p) was solely present in blaCMY-producing E. coli (n=9), within the same genetic environment blaCMY–blc–sugE(p). Other small multidrug efflux pumps were found without association for ESBL-types: emrE (n=5) and the truncated qacEΔ1 (n=18).

Conclusion: Coresistance of ESBL enzymes and SMR efflux pumps in E. coli was common and might indicate that other substances than antibiotics contribute to the spread and emergence of antibiotic resistance. Keywords: biocides, Escherichia coli, extended-spectrum β-lactamases, quaternary ammonium compounds.

Keywords: biocides, Escherichia coli, extended-spectrum β-lactamases, quaternary ammonium compounds.

How to cite this article: Sütterlin S, Heydecke A, Tano E (2020) Coresistance to quaternary ammonium compounds in extended-spectrum beta-lactamase-producing Escherichia coli, Int. J. One Health, 6(2): 134-142.

Received: 06-05-2020 Accepted: 17-08-2020 Published online: 15-09-2020

Corresponding author: Susanne Sütterlin E-mail: susanne.sutterlin@kbh.uu.se

DOI: 10.14202/IJOH.2020.134-142

Copyright: Sütterlin, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.