Prevalence of multidrug resistance and extended-spectrum beta-lactamase-producing Klebsiella pneumoniae from dairy cattle farm wastewater in East Java Province, Indonesia

Research Article

International Journal of One Health, 9(2): 141-149

https://doi.org/10.14202/IJOH.2023.141-149

Fidi Nur Aini Eka Puji Dameanti^1,2, Sheila Marty Yanestria³, Agus Widodo⁴, Mustofa Helmi Effendi⁵, Hani Plumeriastuti⁶, Wiwiek Tyasningsih⁷, Emmanuel Nnabuike Ugbo⁸, Rahayu Sutrisno², and M. Ali Akram Syah⁹

1. Doctoral Student of Sains Veteriner, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Mulyorejo, Kampus C FKH-UNAIR, Surabaya 60115, East Java, Indonesia.
2. Laboratory of Microbiology and Immunology Veterinary, Faculty of Veterinary Medicine, Universitas Brawijaya. Jl. Puncak Dieng, Kalisongo, Malang Regency 65151, East Java, Indonesia.
3. Department of Veterinary Public Health, Wijaya Kusuma Surabaya University, Jl. Dukuh Kupang XXV No.54, Dukuh Kupang, Dukuhpakis, Surabaya, 60225, East Java, Indonesia.
4. Department of Health, Faculty of Vocational Studies, Universitas Airlangga, Jl. Dharmawangsa Dalam Selatan No.28 - 30, Airlangga, Kec. Gubeng, Surabaya, 60286, East Java, Indonesia.
5. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
6. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
7. Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
8. Department of Applied Microbiology, Faculty of Science, Ebonyi State University, 480211, Abakaliki, Nigeria.
9. Professional student on Faculty of Veterinary Medicine, Universitas Brawijaya, Jl. Puncak Dieng, Kalisongo, Malang Regency 65151, East Java, Indonesia.

Background and Aim: Antibiotic resistance in Klebsiella pneumoniae, especially extended-spectrum beta-lactamase (ESBL) producers, has become a global public health problem. This study aimed to estimate the prevalence of multidrug resistance (MDR) and ESBL-producing K. pneumoniae in wastewater from dairy farms.

Materials and Methods: This study was conducted on dairy farms in East Java Province from June to October 2022. In total, 342 dairy farm wastewater samples were isolated on buffered peptone water media from six cities/regencies with the highest dairy cattle population in East Java. Samples were identified using MacConkey agar media, Gram-staining,eosin-methylene blue agar, and biochemical tests. In total, 14.32% (49/342) samples contained K. pneumoniae. Positive isolates were tested for antibiotic sensitivity. Klebsiella pneumoniae resistant to beta-lactam was confirmed using the double-disk synergy test to confirm the presence of ESBL-producing bacteria.

Results: The percentage of antibiotic resistance in K. pneumoniae was 98% resistance to ampicillin, 67.3% to cefotaxime, 46.9% to tetracycline, 49% to ciprofloxacin, 98% to streptomycin, 14.3% to sulfamethoxazole–trimethoprim, and 83.7% to chloramphenicol. The prevalence of MDR in K. pneumoniae was 12.57% (43/342), with the highest prevalence in the five classes of antibiotics at 41.86% (18/43), and the prevalence of ESBL-producing K. pneumoniae was 5.55% (19/342), with the highest prevalence in the districts of Blitar and Pasuruan at 26.31% (5/19).

Conclusion: Although the prevalence of ESBL-producing K. pneumoniae in wastewater samples from dairy farms was low, caution is recommended because they can be a reservoir for ESBL. Keywords: animal health, dairy cattle, extended-spectrum beta-lactamase, human health, Klebsiella pneumoniae, wastewater.

Keywords: animal health, dairy cattle, extended-spectrum beta-lactamase, human health, Klebsiella pneumoniae, wastewater.

How to cite this article: Dameanti FNAEP, Yanestria SM, Widodo A, Effendi MH, Plumeriastuti H, Tyasningsih W, Ugbo EN, Sutrisno R, and Akram Syah MA (2023) Prevalence of multidrug resistance and extended-spectrum beta-lactamase-producing Klebsiella pneumoniae from dairy cattle farm wastewater in East Java Province, Indonesia, Int. J. One Health, 9(2): 141-149.

Received: 20-07-2023 Accepted: 11-10-2023 Published online: 21-11-2023

Corresponding author: Mustofa Helmi Effendi E-mail: mhelmieffendi@gmail.com

DOI: 10.14202/IJOH.2023.141-149

Copyright: Dameanti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.