Detection and molecular characterization of multiresistant Enterobacteriaceae carried by houseflies in the city of Bobo-Dioulasso, Burkina Faso

Research Article

International Journal of One Health, 10(1): 12-19

https://doi.org/10.14202/IJOH.2024.12-19

Sanou D. M. Soufiane¹, Yerbanga R. Serge^2,3, Bangre Tinlé², N’Do Sévérin³, Kaboré N. Firmin¹, Traoré Isidore¹, Zoungrana Jacques⁴, Yaméogo Inès², Nikiéma Félicité², Fofana Aminata³, Batiéné Naomi², Ouédraogo Jean-Bosco², Sangaré Ibrahim⁴, and Sanou Mahamoudou⁵

1. Centre MURAZ, Laboratoire de Biologie clinique, Bobo dioulaso, Burkina Faso.
2. Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso.
3. Department of Microbiology, Institute of Research in Health Sciences, Western Regional Direction, Bobo Dioulasso, Burkina Faso.
4. Department of Pharmacy, Nazi BONI University, INSSA, Bobo Dioulasso, Burkina Faso.
5. Department of Pharmacy, Joseph KI-ZERBO University, UFR/SDS, Ouagadougou, Burkina Faso.

Background and Aim: Houseflies (Musca domestica) are synanthropic insects that are vectors of a wide range of multidrug-resistant pathogens responsible for infectious diseases. The aim of this study was to detect and characterize multidrug-resistant Enterobacteriaceae carried by houseflies in Bobo-Dioulasso.

Materials and Methods: A total of 500 houseflies were captured in hospital and non-hospital environments in the city of Bobo-Dioulasso. For bacteriological analysis, they were divided into 125 batches of five flies each. Multidrug-resistant bacteria isolated on MacConkey agar supplemented with 4 μg/mL cefotaxime were identified on the basis of biochemical characteristics. Antibiotic susceptibility profiles were determined using the agar diffusion method. blaCTX-M resistance genes and quinolone resistance genes (plasmid-mediated quinolone resistance) were detected by conventional polymerase chain reaction.

Results: Among 115 bacterial strains obtained, 26 were extended-spectrum beta-lactamase (ESBL)-producing enterobacteria: Escherichia coli (15), Klebsiella pneumoniae (6), Enterobacter cloacae (4), and Morganella morganii (1). Carriers were statistically more important in hospitals (12/26, p = 0.03). No carbapenem-resistance strains were observed. We identified ESBL resistance genes (Cefotaximase Munich; CTX-M group 1) (25/26) and quinolone resistance genes (QnrS) (6/26).

Conclusion: Houseflies in the city of Bobo-Dioulasso are vectors for the transmission of multidrug-resistant enterobacteria. There is a need to monitor the associated risks for public health. Keywords: Bobo-Dioulasso, extended-spectrum beta-lactamase, Gram-negative bacilli, housefly, multiresistant Enterobacteriaceae.

Keywords: Bobo-Dioulasso, extended-spectrum beta-lactamase, Gram-negative bacilli, housefly, multiresistant Enterobacteriaceae.

How to cite this article: Soufiane SDM, Serge YR, Tinlé B, Séverin N, Firmin KN, Isidore T, Jacques Z, Inès Y, Félicité N, Aminata F, Naomie B, Jean-Bosco O, Ibrahim S, and Mahamoudou S (2024) Detection and molecular characterization of multiresistant Enterobacteriaceae carried by houseflies in the city of Bobo-Dioulasso, Burkina Faso, Int. J. One Health, 10(1): 12–19.

Received: 16-07-2023 Accepted: 12-12-2023 Published online: 12-01-2024

Corresponding author: Sanou D. M. Soufiane E-mail: domalick2000@yahoo.fr

DOI: 10.14202/IJOH.2024.12-19

Copyright: Soufiane, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.