Open Access
Research (Published online: 12-01-2024)
2. Detection and molecular characterization of multiresistant Enterobacteriaceae carried by houseflies in the city of Bobo-Dioulasso, Burkina Faso
Sanou D. M. Soufiane, Yerbanga R. Serge, Bangre Tinlé, N’Do Sévérin, Kaboré N. Firmin, Traoré Isidore, Zoungrana Jacques, Yaméogo Inès, Nikiéma Félicité, Fofana Aminata, Batiéné Naomi, Ouédraogo Jean-Bosco, Sangaré Ibrahim, and Sanou Mahamoudou
International Journal of One Health, 10(1): 12-19

Sanou D. M. Soufiane: Centre MURAZ, Laboratoire de Biologie clinique, Bobo dioulaso, Burkina Faso.
Yerbanga R. Serge: Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso; Department of Microbiology, Institute of Research in Health Sciences, Western Regional Direction, Bobo Dioulasso, Burkina Faso.
Bangre Tinlé: Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso.
N’Do Sévérin: Department of Microbiology, Institute of Research in Health Sciences, Western Regional Direction, Bobo Dioulasso, Burkina Faso.
Kaboré N. Firmin: Centre MURAZ, Laboratoire de Biologie clinique, Bobo dioulaso, Burkina Faso.
Traoré Isidore: Centre MURAZ, Laboratoire de Biologie clinique, Bobo dioulaso, Burkina Faso.
Zoungrana Jacques: Department of Pharmacy, Nazi BONI University, INSSA, Bobo Dioulasso, Burkina Faso.
Yaméogo Inès: Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso.
Nikiéma Félicité: Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso.
Fofana Aminata: Department of Microbiology, Institute of Research in Health Sciences, Western Regional Direction, Bobo Dioulasso, Burkina Faso.
Batiéné Naomi: Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso.
Ouédraogo Jean-Bosco: Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso.
Sangaré Ibrahim: Department of Pharmacy, Nazi BONI University, INSSA, Bobo Dioulasso, Burkina Faso.
Sanou Mahamoudou: Department of Pharmacy, Joseph KI-ZERBO University, UFR/SDS, Ouagadougou, Burkina Faso.

doi: 10.14202/IJOH.2024.12-19

Article history: Received: 16-07-2023, Accepted: 12-12-2023, Published online: 12-01-2024

Corresponding author: Sanou D. M. Soufiane

E-mail: domalick2000@yahoo.fr

Citation: Soufiane SDM, Serge YR, Tinlé B, Séverin N, Firmin KN, Isidore T, Jacques Z, Inès Y, Félicité N, Aminata F, Naomie B, Jean-Bosco O, Ibrahim S, and Mahamoudou S (2024) Detection and molecular characterization of multiresistant Enterobacteriaceae carried by houseflies in the city of Bobo-Dioulasso, Burkina Faso, Int. J. One Health, 10(1): 12–19.
Abstract

Background and Aim: Houseflies (Musca domestica) are synanthropic insects that are vectors of a wide range of multidrug-resistant pathogens responsible for infectious diseases. The aim of this study was to detect and characterize multidrug-resistant Enterobacteriaceae carried by houseflies in Bobo-Dioulasso.

Materials and Methods: A total of 500 houseflies were captured in hospital and non-hospital environments in the city of Bobo-Dioulasso. For bacteriological analysis, they were divided into 125 batches of five flies each. Multidrug-resistant bacteria isolated on MacConkey agar supplemented with 4 μg/mL cefotaxime were identified on the basis of biochemical characteristics. Antibiotic susceptibility profiles were determined using the agar diffusion method. blaCTX-M resistance genes and quinolone resistance genes (plasmid-mediated quinolone resistance) were detected by conventional polymerase chain reaction.

Results: Among 115 bacterial strains obtained, 26 were extended-spectrum beta-lactamase (ESBL)-producing enterobacteria: Escherichia coli (15), Klebsiella pneumoniae (6), Enterobacter cloacae (4), and Morganella morganii (1). Carriers were statistically more important in hospitals (12/26, p = 0.03). No carbapenem-resistance strains were observed. We identified ESBL resistance genes (Cefotaximase Munich; CTX-M group 1) (25/26) and quinolone resistance genes (QnrS) (6/26).

Conclusion: Houseflies in the city of Bobo-Dioulasso are vectors for the transmission of multidrug-resistant enterobacteria. There is a need to monitor the associated risks for public health.

Keywords: Bobo-Dioulasso, extended-spectrum beta-lactamase, Gram-negative bacilli, housefly, multiresistant Enterobacteriaceae.