Research Article | 28 Nov 2017

Mingling of human and veterinary strains of Staphylococcus aureus: An emerging issue in health-care systems

Sara Giordana Rimoldi1, Annamaria Di Gregorio1, Vittorio Sala2, Eleonora De Faveri2, Cristina Pagani1, Pietro Olivieri3, Claudio Savi4, Anna Lisa Ridolfo5, Antona Carlo6, and Maria Rita Gismondo1Show more
1. Laboratory of Microbiology, Virology and Bioemergency, ASST Fatebenefratelli Sacco-Polo Universitario, Via G.B. Grassi 74, 20157 Milan, Italy.
2. Department of Veterinary Sciences and Public Health, Universita di Milano, Italy.
3. Medical Management Staff, ASST Fatebenefratelli Sacco-Polo Universitario, Via G.B. Grassi 74, 20157 Milan, Italy.
4. Cardiac Surgical Intensive Care Unit, ASST Fatebenefratelli Sacco-Polo Universitario, Via G.B. Grassi 74, 20157 Milan, Italy.
5. Department of Infectious Diseases, ASST Fatebenefratelli Sacco-Polo Universitario, Via G.B. Grassi 74, 20157 Milan, Italy.
6. Cardiac Surgery Unit, ASST Fatebenefratelli Sacco-Polo Universitario, Via G.B. Grassi 74, 20157 Milan, Italy.

Corresponding author: Sara Giordana Rimoldi (sara.rimoldi@asst-fbf-sacco.it)

Received: 03-08-2017, Accepted: 30-10-2017, Published: 28-11-2017

INTERNATIONAL JOURNAL OF ONE HEALTH | pg no. 77-82 | Vol. 3, Issue 1 | DOI: 10.14202/IJOH.2017.77-82
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Abstract

              
Background and Aim: Methicillin-resistant Staphylococcus aureus remains a leading cause of hospital and community infections. We report a retrospective molecular characterization of S. aureus strains from different settings: hospital workers and patients, and veterinarian surgeons and pets.
Materials and Methods: Eighty-nine S. aureus isolates obtained from nasal swabs of 10 patients, 17 health-care workers (HCWs), 9 pets, and 53 veterinarians were genotypically characterized by means of repetitive extragenic palindromic polymerase chain reaction (Rep PCR) and whole-genome sequencing.
Results: Thirteen different sequence types (STs) were detected: ST398, ST22, ST8, ST30, ST15, ST5, ST121, ST45, ST10, ST6, ST34, ST97, and ST1. Two new STs differing from ST22 and ST5 for a single multilocus sequence typing gene were also identified. Rep PCR documented a genetic relationship among isolates obtained from 5 veterinarians and 10 HCWs.
Conclusion: The large diversity of S. aureus strains detected may reflect a larger epidemiology within the hospital and community, in which companion animals likely act as a reservoir. We identified the circulation of ST5, ST8, ST15, ST22, ST30, ST45, and ST121 both in the hospital and veterinarian environment. Starting from the idea of a unique setting where our population lives, we consider the relationship between community- and hospital-acquired S. aureus. Keywords: health-care workers, multilocus sequence typing, S. aureus, single-nucleotide polymorphisms, pets, veterinarians..

             
Keywords: health-care workers, multilocus sequence typing, S. aureus, single-nucleotide polymorphisms, pets, veterinarians..