Research Article | 07 Dec 2021

Prevalence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics, Surabaya, Indonesia

Luviana Kristianingtyas1, Mustofa Helmi Effendi2, Adiana Mutamsari Witaningrum2, Dhandy Koesoemo Wardhana2, and Emmanuel Nnabuike Ugbo3Show more
1. Department of Veterinary Public Health, Postgraduate Student on Veterinary Public Health Study, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia.
2. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia.
3. Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, Ebonyi State, Nigeria.

Corresponding author: Mustofa Helmi Effendi (mheffendi@yahoo.com)

Received: 11-07-2021, Accepted: 18-10-2021, Published: 07-12-2021

INTERNATIONAL JOURNAL OF ONE HEALTH | pg no. 232-236 | Vol. 7, Issue 1 | DOI: 10.14202/IJOH.2021.232-236
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Abstract

              
Background and Aim: The practice of keeping animals as pets is becoming increasingly common. The upsurge of extended-spectrum β-lactamase (ESBL)-producing organisms of animal origin is a health threat globally. This study aimed to identify the presence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics in Surabaya, Indonesia.
Materials and Methods: A total of 85 rectal swab samples were collected from companion dogs at five animal clinics in different regions of Surabaya, Indonesia. The presence of E. coli was identified from the samples using standard methods, followed by antibiotic sensitivity testing. The resistant isolates were examined for the presence of ESBL using the double-disk synergy test method. The phenotypically identified ESBL-producing E. coli was further confirmed with an automated system using Vitek-2.
Results: The rectal swab samples (n=85) tested were 100% positive for E. coli isolates. Eight (9.41%) out of the 85 E. coli obtained from rectal swabs were extended-spectrum β-lactamase producers. All eight ESBL-producing E. coli were identified by automated Vitek-2 confirmatory tests.
Conclusion: This study provides insight into the prevalence of ESBL-producing organisms isolated from companion dogs in Indonesia. This work indicates the need for the general public to be more aware of the role of companion animals in disseminating pathogenic organisms, since they serve as potential reservoirs in the spread of antibiotic resistance affecting human health. Keywords: animal clinics, companion dogs, extended-spectrum β-lactamase, Escherichia coli, human health.

             
Keywords: animal clinics, companion dogs, extended-spectrum β-lactamase, Escherichia coli, human health.