Prevalence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics, Surabaya, Indonesia

Research Article

International Journal of One Health, 7(2): 232-236

https://doi.org/10.14202/IJOH.2021.232-236

Luviana Kristianingtyas¹, Mustofa Helmi Effendi², Adiana Mutamsari Witaningrum², Dhandy Koesoemo Wardhana², and Emmanuel Nnabuike Ugbo³

1. Department of Veterinary Public Health, Postgraduate Student on Veterinary Public Health Study, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia.
2. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia.
3. Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, Ebonyi State, Nigeria.

Background and Aim: The practice of keeping animals as pets is becoming increasingly common. The upsurge of extended-spectrum β-lactamase (ESBL)-producing organisms of animal origin is a health threat globally. This study aimed to identify the presence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics in Surabaya, Indonesia.

Materials and Methods: A total of 85 rectal swab samples were collected from companion dogs at five animal clinics in different regions of Surabaya, Indonesia. The presence of E. coli was identified from the samples using standard methods, followed by antibiotic sensitivity testing. The resistant isolates were examined for the presence of ESBL using the double-disk synergy test method. The phenotypically identified ESBL-producing E. coli was further confirmed with an automated system using Vitek-2.

Results: The rectal swab samples (n=85) tested were 100% positive for E. coli isolates. Eight (9.41%) out of the 85 E. coli obtained from rectal swabs were extended-spectrum β-lactamase producers. All eight ESBL-producing E. coli were identified by automated Vitek-2 confirmatory tests.

Conclusion: This study provides insight into the prevalence of ESBL-producing organisms isolated from companion dogs in Indonesia. This work indicates the need for the general public to be more aware of the role of companion animals in disseminating pathogenic organisms, since they serve as potential reservoirs in the spread of antibiotic resistance affecting human health. Keywords: animal clinics, companion dogs, extended-spectrum β-lactamase, Escherichia coli, human health.

Keywords: animal clinics, companion dogs, extended-spectrum β-lactamase, Escherichia coli, human health.

How to cite this article: Kristianingtyas L, Effendi MH, Witaningrum AM, Wardhana DK, Ugbo EN (2021) Prevalence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics, Surabaya, Indonesia, Int J One Health, 7(2): 232-236.

Received: 11-07-2021 Accepted: 18-10-2021 Published online: 07-12-2021

Corresponding author: Mustofa Helmi Effendi E-mail: mheffendi@yahoo.com

DOI: 10.14202/IJOH.2021.232-236

Copyright: Kristianingtyas, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.