New phenomena for clinicians, model of Candida albicans mobilization before and after biofilm formation in the intestinal mucosa of Wistar rats (Rattus norvegicus)

Research Article

International Journal of One Health, 7(2): 165-170

https://doi.org/10.14202/IJOH.2021.165-170

Masfufatun Masfufatun¹, Loo Hariyanto Raharjo¹, Harsono Wiradinata¹, Putu Oky Ari Tania², Ni'matuzahroh Ni'matuzahroh³, and Afaf Baktir⁴

1. Department of Biochemistry, Faculty of Medicine, University of Wijaya Kusuma Surabaya, Surabaya, Indonesia.
2. Department of Biomedicine and Biomolecular, Faculty of Medicine, University of Wijaya Kusuma Surabaya, Surabaya, Indonesia.
3. Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.
4. Department of Chemistry, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.

Background and Aim: The virulence and antifungal resistance of Candida albicans are recently known for their ability to form biofilm. This research aimed to construct an in vivo model of C. albicans biofilm in Wistar rats' intestinal mucosa and study their mobilization while in a planktonic and biofilm formation. In this study, there was one treatment group that was treated with three antibiotics, immunosuppressants, and C. albicans.

Materials and Methods: This study was divided into control and treatment groups. The data sampling was conducted after C. albicans inoculation. The C. albicans biofilm formation stage was monitored with colony-forming units method calculation every week post-inoculation and then observed by the confocal laser scanning microscope.

Results: The planktonic C. albicans overgrowth occurred up to 14 days after inoculation. The formation and maturation of C. albicans biofilm in the intestinal mucosa started in the 28th and 35th-day post-inoculation, respectively. The density of planktonic C. albicans in the stool was dramatically decreased on the 35th day. Before the biofilm formation, the planktonic Candida was carried away by food scraps to be released as a stool. However, there were minuscule or no planktonic Candida observed in the stool during and after biofilm formation. Instead, they were attached to the caecum's mucosa as a biofilm.

Conclusion: We have proved that the planktonic C. albicans with its mobile nature were carried into the stool along with the rest of the feed, as we observed a lot of C. albicans cells found in the stool. Meanwhile, on day 28 after administration of antibiotics and immunosuppressants, no C. albicans was found in the stool samples, and at the same time, we observed C. albicans cells and their matrix attached to the intestinal mucosa as a biofilm. Keywords: biofilm, candidiasis, Candida albicans, confocal laser scanning microscope, intestinal mucosa.

Keywords: biofilm, candidiasis, Candida albicans, confocal laser scanning microscope, intestinal mucosa.

How to cite this article: Masfufatun M, Raharjo LH, Wiradinata H, Tania POA, Ni'matuzahroh N, Baktir A (2021) New phenomena for clinicians, model of Candida albicans mobilization before and after biofilm formation in the intestinal mucosa of Wistar rats (Rattus norvegicus), Int. J. One Health, 7(2): 165-170.

Received: 10-11-2020 Accepted: 10-05-2021 Published online: 23-08-2021

Corresponding author: Afaf Baktir E-mail: afafi2001@yahoo.com

DOI: 10.14202/IJOH.2021.165-170

Copyright: Masfufatun, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.